Skip to main content
Fig. 7 | Alzheimer's Research & Therapy

Fig. 7

From: Attenuation of Alzheimer’s brain pathology in 5XFAD mice by PTH1-34, a peptide of parathyroid hormone

Fig. 7

More PTH1-34 association with astrocytes in 5XFAD brain than that of WT control. A Schematic diagram of experimental design. 2.5 ~ MO WT and 5XFAD female mice were administered PTH1-34-Biotin (100 μg/100 μl) or vehicle (phosphate buffer, 100 μl) by tail-intravenous injection to detect PTH1-34 diffusion in vivo. Mice were sacrificed after 30 min for brain isolation and tissue sectioning, and immunofluorescence staining was used to analyze the distribution of PTH1-34-Biotin. B Representative images and high-magnification images of Biotin (green) co-immunostaining with GFAP, IBA1, SLC16A1, and MAP2 (red) respectively from 5XFAD + Veh, 5XFAD + PTH1-34-Biotin and WT + PTH1-34-Biotin female mice. All images were obtained from the cortex regions. Scale bars were indicated in the panel. C Quantification of relative Biotin fluorescence intensity in these three groups (mean ± SD; n = 8 mice per group). **P < 0.01, ***P < 0.001, one-way ANOVA with Kruskal–Wallis multiple-comparison test. D Quantification of PTH1-34-Biotin distribution in 5XFAD brain cells from B. The distribution of PTH1-34 in various cells was shown as a percentage, with a total proportion of 100%, derived from the mean value of data collected from all mice in the 5XFAD + PTH1-34-Biotin group

Back to article page