Fig. 2

Early activation of TLR3 reduced anxiety and depression and increased spontaneous activity in AD mice. A Representative track plots in the OFT. B The ratio of the time spent in the inner zone versus the outer zone was reduced in AD mice when compared with WT control mice. Poly (I:C) treatment increased the ratio of the time spent in the inner zone versus the outer zone compared with AD control mice. C The total travelled distance of AD + Saline mice was shorter than that of WT + Saline mice. Compared with the AD + Saline mice, the total travelled distance of the AD + Poly(I:C) mice was longer. D The distance covered in the central area by AD + Saline mice was shorter than that by WT + Saline mice. Moreover, the distance covered in the central area of AD + Poly(I:C) mice was longer than that of AD + Saline mice. E The number of excrements in the AD + Saline mice was greater than that in the WT + Saline mice and less than that in the AD + Poly(I:C) mice. F In the TST, the resting immobility time was longer in AD + Saline mice and AD + Poly(I:C) mice than in the WT + Saline mice and longer in AD + Saline mice than in AD + Poly(I:C) mice. G In the EPM, the time in the enclosed arms did not show a significant difference among the groups (#: WT + Saline vs. AD + Saline, P < 0.05; &: WT + Saline vs. AD + Poly(I:C), P < 0.05; *: AD + Saline vs. AD + Poly(I:C), P < 0.05; n = 8–12/group)