Experiment 1 | Experiment 2 | Number of shared significant pathways (overlap coefficient) | Summary of processes | Relevance to AD |
---|---|---|---|---|
hAD Tau seeded RCNs, day 14 | Unseeded RCNs, day 14 | 47 (0.59) | GPCR signalling Extracellular matrix (ECM) organisation Cholesterol homeostasis Inflammation Angiogenesis | Mediates both the inflammatory response in microglia and modulation of calcium signalling [26, 27] Reduction in hippocampal ECM reverses memory deficits associated with AD [28] Regulates both tau and amyloid pathology by independent mechanisms [29] Causative of and aggravated by tau pathology [30] Tau pathology induces blood vessel abnormalities [31] |
hAD Tau seeded RCNs, day 7 | Unseeded RCNs, day 7 | 4 (0.29) | Metabolism | Disruptions in metabolic processes linked to AD progression [32,33,34] |
hAD Tau seeded RCNs, day 3 | Unseeded RCNs, day 3 | 0 (0.00) | - | - |