Fig. 6

Analysis of action potential–independent mEPSCs and mIPSCs reveals enhanced frequencies in about 8-month-old 5xFAD, htau-KI mice, and 5xFADxhtau-KI. a Mean amplitude of mEPSCs is unchanged in the three genetically modified mouse strains as compared to WT (See insets in a). Mean ± SEM. WT (n=41), 5xFAD (n=38), htau-KI (n=43), 5xFADxhtau-KI (n=38). b Mean frequency of mEPSCs of htau-KI mice is significantly increased compared to WT controls, but the increased means in 5xFAD and 5xFADxhtau-KI mice fail to reach statistical significance (See insets in b). Mean ± SEM. WT (n=41), 5xFAD (n=38), htau-KI (n=43), 5xFADxhtau-KI (n=38). c mEPSC half-width, a measure that characterizes the kinetics of inactivation does not differ across genotypes. Analyses of the probability distributions of the data (a–c) revealed significant genotype differences for all three parameters. Mean ± SEM. WT (n=40), 5xFAD (n=37), htau-KI (n=42), 5xFADxhtau-KI (n=37). d Similar to the mean mEPSC amplitudes (inset in a), the mean mIPSC amplitudes do not differ between genotypes (inset in d). Mean ± SEM. WT (n=42), 5xFAD (n=38), htau-KI (n=43), 5xFADxhtau-KI (n=38). e Frequency of mIPSCs in htau-KI and 5xFADxhtau-KI mice is markedly increased (inset in e). Mean ± SEM. WT (n=42), 5xFAD (n=38), htau-KI (n=43), 5xFADxhtau-KI (n=38). f Kinetics of mIPSC inactivation as measured by the half-width was very similar in all genotypes (inset in f). Kolmogorov-Smirnov tests yielded significant genotype differences for the amplitude, the frequency, and the half-width of mIPSCs (d–f). Mean ± SEM. WT (n=42), 5xFAD (n=38), htau-KI (n=43), 5xFADxhtau-KI (n=38). Statistics: Welch’s one-way ANOVA test using the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli. *P < 0.05, **P < 0.01. Cumulative distribution functions of mEPSCs and mIPSCs were compared by Kolmogorov-Smirnov test