Fig. 4

Analysis of tau and Aβ pathology. a,b Aβ staining using 3A1 antibody in representative sagittal brain slices of female, 13-month-old 5xFAD (a) and 5xFADxhtau-KI (b) mice. Scale 500 μm. c Quantification of the occupied total plaque area in brain slices analogous to a and b in female (F) and male (M) 13-month-old 5xFAD and 5xFADxhtau-KI mice. Plaque area in WT and htau-KI mice was equal to zero (not shown). Mean±SEM. n=5–8. * P < 0.05 unpaired t-test. d Sections from the hypothalamic region of 13-month-old, female 5xFAD and 5xFADxhtau-KI mice showing Congo red-stained Aβ plaques surrounded by dystrophic neurites (arrow heads). Three different antibodies were used for DAB staining of tau protein: CP13 (pathologic tau), PHF1 (later stage tangles), and MC1 (conformation-specific). Note that MC1-positive tau occurs exclusively in 5xFADxhtau-KI. Scale 20 μm. e Quantification of total and MC1-surrounded plaques in the hypothalamus of female 5xFAD and 5xFADxhtau-KI mice of 7 months (7 M) and 13 months (13 M) of age. Mean ± SD. 5xFAD, 7 M (n=9); 5xFAD, 13 M (n=6); 5xFADxhtau-KI, 7 M (n=7); 5xFADxhtau-KI, 7 M (n=6). f Quantification of total and MC1-surrounded plaques in the inferior colliculus of female 5xFAD and 5xFADxhtau-KI mice of 7 months (7 M) and 13 months (13 M) of age. Mean ± SD. 5xFAD, 7 M (n=8); 5xFAD, 13 M (n=5); 5xFADxhtau-KI, 7 M (n=7); 5xFADxhtau-KI, 7 M (n=6)