Fig. 9
Amyloid pathology, microgliosis, and ChAT+ cells in the medial septum-diagonal band (MSDB) complex of the basal forebrain of 12-month-old mice. A Photomicrographs of amyloid plaques stained with methoxy-XO4 (green) in MSDB complex. B Photomicrographs of immunostaining of activated IBA-1 (red) in MSDB complex. C Photomicrographs of immunohistochemistry staining of choline acetyl transferase (ChAT) in MSDB complex. ChAT, a cholinergic marker stained with monoclonal rabbit anti-ChAT antibody (red); DAPI, stained the nuclei (blue). Scale bar, 500 μm for whole sections. D Corresponding distributions of plaque size by total number of plaques. E Total amyloid plaques number in MSDB complex. F Percent amyloid plaque area in MSDB complex. G Activated IBA-1 number in MSDB complex. H Activated IBA-1 percent area in MSDB complex. I Quantification of ChAT in MSDB complex. Data is presented as mean±SEM. *p < 0.05, **p < 0.01, ***p < 0.001 are considered as statistically significant. $, as compared to APP-NE group; b, as compared to control group; c, as compared to APP-NE group. Control group, C57BL/6 (n = 5). APP-NE group, APPNL-G-F with no exercise (n = 4). APP-Ex group, APPNL-G-F mice exposed to exercise (n = 4)