Fig. 1
nEV Aβ concentrations in different diagnostic groups and ROC curves. Analyses performed after correcting the particle numbers to 3 × 108 for nEV Aβ40 (A) and nEV Aβ42 (B). C The ratio of Aβ42 to Aβ40 among the different diagnostic groups. Statistical analysis was conducted using the Kruskal–Wallis H test, followed by multiple post hoc comparisons (adjusted p value). Comparisons among the groups: *p < 0.05; **p < 0.01; ***p < 0.001; ns, > 0.05. Furthermore, correlation analysis was performed between Aβ42 and Aβ40 (D), and the correlation coefficients and p values are listed. E ROC curves used to distinguish Aβ− NCs from Aβ+ NCs (red), aMCI (green), and ADD (blue) using nEV Aβ42 levels. F We attempted to use different combinations to distinguish Aβ− NCs from Aβ+ NCs. AUC and its corresponding 95% CIs and different diagnostic combinations are listed. All outliers were excluded; outliers were defined as less than Q1 − 2.5 × IQR or greater than Q3 + 2.5 × IQR. Aβ, β-amyloid; NCs, cognitively normal controls; aMCI, amnestic mild cognitive impairment; ADD, Alzheimer’s disease dementia; nEV, neuronal-derived extracellular vesicle; AUC, area under the curve; ROC, receiver operating characteristic; CI, confidence interval; IQR, inter-quartile range; Q1, lower quartile; Q3, upper quartile