Fig. 6From: RETRACTED ARTICLE: Preservation of dendritic spine morphology and postsynaptic signaling markers after treatment with solid lipid curcumin particles in the 5xFAD mouse model of Alzheimer’s amyloidosisSLCP treatment partially preserved synaptophysin and PSD95 levels in the PFC and hippocampus of 5xFAD mice. Six- and 12-month-old 5xFAD and age-matched controls were treated with SLCP (100 mg/kg) for 2 months and then their brains were extracted, sectioned coronally at 40 μm, and stained with anti-synaptophysin and PSD95 antibodies. Images were taken using a fluorescent microscope at 4x0 (total magnification = 400x). a, b The vehicle-treated 5xFAD mice showed a decrease in synaptophysin fluorescent signals in the cortex, as well as the CA1, and CA3 areas of the hippocampus when compared to WT and SLCP-treated mice. e–h Western blot data from cortical and hippocampal tissue showed that synaptophysin was significantly reduced in vehicle-treated 5xFAD mice and that SLCP treatment prevented much of these losses. Scale bar= 100 μm and applicable to all other images. c, d Immunofluorescent intensity was decreased in vehicle-treated 5xFAD mice in comparison to WT mice, but SLCP treatments moderately preserved these levels in all three of the brain regions sampled. e, f and i, j Western blot data from cortical and hippocampal tissue showed that PSD95 levels were significantly reduced in vehicle-treated 5xFAD mice and that SLCP treatment partially preserved these levels. Scale bar indicates 100 μm and applicable to all images. *p < 0.05, **p < 0.01 in comparison to WT + vehicle, 5xFAD + SLCP, and WT + SLCP. Scale bar = 100 μm for all images.Back to article page