Fig. 3From: RETRACTED ARTICLE: Preservation of dendritic spine morphology and postsynaptic signaling markers after treatment with solid lipid curcumin particles in the 5xFAD mouse model of Alzheimer’s amyloidosisSLCP inhibited Aβ plaque load and amount of Aβ in 5xFAD brain. Six- and twelve-month-old 5xFAD and age-matched control animals were treated with SLCP (100 mg/kg) or vehicle for 2 months, and their brains were perfused with 4% paraformaldehyde. Forty-micron coronal sections were made using a cryostat and were stained with for Aβ using a curcumin-based solution (1 μM, dissolved 70% methanol, 10 min) and the images were taken using a fluorescent microscope with a × 20 objective (total magnification = × 200). a Representative images show that curcumin binds with Aβ, similar to Aβ-specific antibody (6E10). b Representative images of Aβ plaques stained by curcumin in cortex, hippocampus (CA1, CA3, DG, subicular complex), and entorhinal cortex from vehicle- and SLCP-treated 5xFAD mice at both 6- and 12-months of age. c, d Morphometric analysis showed that Aβ plaques were significantly less (*p < 0.05 and **p < 0.01) in all the abovementioned areas of 5xFAD mice treated with SLCP. e Representative Western blot data for Aβ levels from cortical and hippocampal tissue in WT and 5xFAD mice after treatment with SLCP and probe with 6E10. f, g Densitometric analysis revealed that SLCP treatment significantly decreased Aβ levels in 5xFAD mice treated with SLCP in both cortical and hippocampal tissue in both 6- (f) and 12-month (g) mice. Scale bar = 100 μm and is applicable to all images. *p < 0.05, **p < 0.01 in comparison to WT + vehicle, 5xFAD + SLCP, and WT + SLCPBack to article page