Fig. 4
TREM2, β-Catenin, and IL-34 can compensate CSF1R deletion. a Western blot analysis of TREM2/β-Catenin pathway in WT and cKO at 3 months. WT, n = 6, n = 7 for cKO (TREM2, *p = 0.0384, β-Catenin **p = 0.0360, APC, **p = 0.0023, Syndecan-1, *p = 0.0498, IL-34, *p = 0.0274). b Western blot analysis on 6-month-old APP cKO and their littermate controls. APPSwe/PS1 and APP cKO 6-month-old, n = 6 (TREM2, *p = 0.0356, β-Catenin **p = 0.0027, BDNF, **p = 0.0071, Syndecan-1,*p = 0.0498, IL-34,*p = 0.0274, PSD-95,*p = 0.0120). c Western blot analysis on 8-month-old APP cKO and their littermate controls. APPSwe/PS1 and APP cKO 8-month-old, n = 6 (TREM2, *p = 0.0470, β-Catenin, *p = 0.0352, BDNF, *p = 0.0494, Syndecan-1, *p = 0.0378, Bace-1, **p = 0,0010). d Representative image of BACE-1 (green) and 6E10 (red) in APPSwe/PS1 and APP cKO 8-month-old. Scale bar = 50 μm. e Representative image of microglia in cortex area (red) and CX3CR1-GFP (green) in 6-month-old APP and APP cKO mice. CX3CR1 (green)-positive cell in a blood vessel in APP at 6 months. Scale bar = 100 μm