Fig. 2
The deletion of CSF1R does not affect microglia survival and delay cognitive decline in APP cKO mice. a–b Representative image of microglia (Iba1 green) in APPSwe/PS1 (6–8 months old) and APP cKO (6–8 months old). Scale bar = 500 μm and 20 μm. c Unbiased stereological count of microglia in 6–8 months old APPSwe/PS1 and APP cKO. APP 6m, n = 6, APP 8m n = 4, n = 6 APP cKO 6m, and n = 4 APP cKO 8m. d NOR with 3-time points comparison of WT, APPSwe/PS1, and APP cKO from 3 to 8 months (###, ## Discrimination differs from 0.5, APPSwe/PS1 6-month-old ***p = 0.0002, 8-month-old ***p = 0.0007). n = 10 for all groups. e Average time of observation during NOR acquisition phase. f Nesting with 3-time points comparison of WT, APPSwe/PS1, and APP cKO from 3 to 8 months. Score range from 1 (untouched square) to 5 (perfect nest) (APPSwe/PS1 8-month-old ***p = 0.0009). n = 10 in all groups. g Western blot analysis on 6- and 8-month-old APP cKO and their littermate controls. APPSwe/PS1 and APP cKO n = 7 (SMI312 heavy chain, *p = 0.0352). h Unbiased count of microglia per plaque in the hippocampus and cortex. APPSwe/PS1 and APP cKO n = 6 (cortex APP cKO 6 months *p = 0.0311)