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Fig. 4 | Alzheimer's Research & Therapy

Fig. 4

From: Trichostatin A ameliorates Alzheimer’s disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice

Fig. 4

TSA elevated albumin expression in BV2 cells, and albumin inhibited Aβ aggregation and facilitated microglial migration. a Western blots and quantitative analysis for albumin in N2a cells, HT22 cells, and BV2 cells. n = 3 per group. b, c Western blots and quantitative analysis for albumin in BV2 cells treated by TSA with different concentrations and different treatment times. n = 3 per group. d Representative TEM images of Aβ42 aggregation in the presence or absence of mouse albumin. Conditions: Aβ42, 20 μM; mouse albumin, 20 μM; 24 h incubation; 37 °C; constant agitation. Scale bar, 200 nm. e Western blots and f quantitative analysis for neurotoxic Aβ oligomers with 6E10 in presence or absence of Albumin. n = 4 per group. g Schematic diagram of transwell system. BV2 cells were seeded onto 8-μm transwell inserts in 24-well plates in the absence or presence of Aβ42 and/or albumin. h Representative images of the transmigrated DAPI-labeled BV2 cells in a transwell chamber. Scale bar, 10 μm. i Quantification of BV2 cell migration induced by 10 μM Aβ42 and/or 10 μM albumin. n = 3 per group. *p < 0.05, **p < 0.01, and ***p < 0.001

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