Fig. 5
From: Exploring the role of RALYL in Alzheimer’s disease reserve by network-based approaches
Hub genes’ trajectory inference in single-nuclei sequencing of human AD entorhinal cortex. a UMAP visualization showing the clustering of single nuclei, colored by the main five types of brain cells. b UMAP visualization showing the location of 4 hub genes within each cell subcluster of brain cell types; RALYL is highly expressed in AD neurons. c The cell trajectory inference of AD and control in the form of pseudo-time. The trajectory of AD patients is from root to state 1, while control is from root to state 2 over pseudo-time. d The hub gene has different expression patterns in state 1 and state 2; SLC9A6 is highly expressed in state 1 while RALYL and CEP112 are highly expressed in state 2. e Pseudo-time projections of hub genes’ drivers in the AD and control subjects, demonstrating the change in relative expression over pseudo-time for the distinct transcriptional states, with each point representing a single cell. Significance based on differential testing by cluster identification used to generate pseudo-time and adjusted for multiple comparisons