Table 1 Primary antibodies used in this study

43D

Total tau

M

Mono-

0.5 μg/mL WB

Produced in-house

PHF-1

P-tau (Ser396/404)

M

Mono-

1:200 WB

1:500 IF

[39]

AT-8

P-tau (Ser202/T205)

M

Mono-

1:1000 WB

Thermo Fisher Scientific

R134d

Total tau

R

Poly-

1:1000 WB

Produced in-house

GAPDH

GAPDH

R

Poly-

1:1000 WB

Santa Cruz Biotechnology

p-APP

Thr668

R

Poly-

1:1000 WB

Cell Signaling Technology

APP

APP

R

Poly-

1:1000 WB

Generated in our institute

Anti-Aβ

Aβ/APP

R

Mono-

1:200 IF

Cell Signaling Technology

GluR1

GluR1

R

Mono-

1:1000 WB

EMD Millipore

Synaptophysin

Synaptophysin

M

Mono-

1:3000 WB

Millipore

SMI 52

MAP2

M

Mono-

1:4000 WB

Sternberger Monoclonals Inc.

PSD95

PSD95

R

Poly-

1: 1000 WB

Cell Signaling Technology

NR1

NMDAR1

R

Mono-

1:500 WB

ABCAM

GluR 2/3

GluR2 + 3

R

Mono-

1:2000 WB

ABCAM

Synapsin-1

Synapsin 1

R

Poly-

1:40000 WB

Enzo Life Sciences

CREB

CREB

M

Mono-

1:1000 WB

Cell Signaling Technology

p-CREB

Ser133

R

Mono-

1:1000 WB

Cell Signaling Technology

Iba-1

Iba-1

R

Poly-

1:1000 WB

Wako Pure Chemical Industries, Ltd.

SMI22

GFAP

M

Mono-

1:2000 WB

1:1000 IF

Sternberger Monoclonals Inc. (Lutherville, MD,)