Table 1 Primary antibodies used in this study
Antibody | Type | Specificity | Phosphorylation sites | Source/reference |
|---|---|---|---|---|
R134d | Poly- | Tau | ||
pS199 | Poly- | P-tau | Ser199 | Invitrogen |
AT8 | Mono- | P-tau | Ser202/Thr205 | Thermo Fisher Scientific. |
pT212 | Poly- | P-tau | Thr212 | Invitrogen |
pT231 | Poly- | P-tau | Thr231 | Invitrogen |
PHF-1 | Mono- | P-tau | Ser396/404 | Dr. P. Davies [12] |
Synapsin-1 | Poly- | Synapsin-1 | Santa Cruz Biotechnology | |
Synaptophysin | Mono- | Synaptophysin | Millipore | |
PSD95 | Mono- | PSD95 | Cell Signaling Technology | |
GFAP | Mono- | GFAP | Millipore | |
Iba1 | Poly- | Iba1 | Abcam | |
CREB | Poly - | CREB | Cell Signaling Technology | |
p-CREB | Poly- | p-CREB | Ser133 | Cell Signaling Technology |
Doublecortin | Poly- | Doublecortin | Abcam (ab18723) | |
β-Amyloid | Mono- | Aβ | Cell Signaling Technology | |
NeuN | Mono- | NeuN | Millipore | |
GAPDH | Poly- | GAPDH | Santa Cruz Biotechnology |