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Fig. 6 | Alzheimer's Research & Therapy

Fig. 6

From: Young blood plasma reduces Alzheimer’s disease-like brain pathologies and ameliorates cognitive impairment in 3×Tg-AD mice

Fig. 6

Young plasma decreases Aβ pathology in 3×Tg-AD mice. ac Thioflavin-S staining in the subiculum and the hippocampal CA1 region of the mouse brain. a Schematic diagram for the thioflavin-S staining in the subiculum and CA1 region. b Representative images of the thioflavin-S staining. c Quantification of the thioflavin-S-positive amyloid plaque area. dh Aβ staining in the subiculum and frontal cortex. d Schematic diagram for the Aβ staining. e Representative images of Aβ staining in the subiculum and CA1 region. f Quantification of the Aβ-positive amyloid plaque area in the subiculum and CA1 region. g, Representative images of the Aβ staining in the frontal cortex. f Quantification of the Aβ-positive amyloid plaque area in the frontal cortex. Amyloid plaque area was defined with a threshold using Otsu’s arithmetic with black and white in ImageJ. The actual amyloid plaque area was calculated by percent plaque area occupied × the whole image area containing the amyloid plaque, which avoided the bias from selecting the regions. The average of the plaque areas from 8 sections (every 6th serial brain sections) of each mouse was used for analysis. Unpaired t test was used for c, f, and h, **p < 0.01. N = 11 for vehicle (saline) control. N = 10 for young plasma treatment

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