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Fig. 8 | Alzheimer's Research & Therapy

Fig. 8

From: A selective p38α/β MAPK inhibitor alleviates neuropathology and cognitive impairment, and modulates microglia function in 5XFAD mouse

Fig. 8

NJK14047 reduces LPS-stimulated microglial neurotoxicity rather than LPS-stimulated astrocyte neurotoxicity. a Scheme of the experiment. Mouse primary microglia and astrocytes were seeded on 6-well plate at 5 × 105 cells/well and stimulated with 50 ng/ml LPS alone or after 2 h of pre-treatment with 10 μM NJK14047. After 22 h of LPS stimulation, all media were changed to fresh neurobasal medium where cells were incubated for 24 h. The conditioned medium was collected to treat primary neurons seeded on 24-well cover glass for TUNEL assay. b Viability of mouse primary neurons incubated with MCM and ACM for 24 h. O.D. at 570 nm were normalized to the control group and shown as percentage of control mean ± SD (n = 3, Kruskal-Wallis test). c TUNEL assay for mouse primary neurons stimulated with mouse primary microglia- and astrocyte-conditioned media. Apoptotic cells were quantified using four images in each cover glass. Data are shown as mean ± SEM (scale bar = 100 μm, n = 5, one-way ANOVA). #P < 0.05 vs. the control group; *P < 0.05 vs. the LPS group

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