Fig. 5

NgR changes APP and its proteolytic amyloidogenic fragment levels in vivo and in vitro. A (a) Representative images and Western blot analysis of expression levels of sAPPβ, sAPPα, APP, BACE1, β-CTF, or α-CTF in the hippocampus. A (b) Densitometry analysis of protein levels of APP and its proteolytic amyloidogenic fragments. A (c) PCR analysis showing the mRNA expression level of APP in the hippocampus. n = 3–9 male mice/group. B (a, b) Neuroblastoma N2a cells were transfected with plasmids, densitometry analysis of protein levels of APP. C (a, b) Representative bands of the Western blot and densitometry analysis of protein levels of APP by siRNA interference in APPswe/HEK293 cells. D (a, b) Overexpression of NgR by transfecting with plasmids in APPswe/HEK293 cells, densitometry analysis of protein levels of APP and its proteolytic amyloidogenic fragments. D (c, d) Representative micrographs of PCR and densitometry analysis of APP. Data are presented as mean ± SEM. n = 3–6. The statistical analysis was performed by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001