Skip to main content
Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Effector function of anti-pyroglutamate-3 Aβ antibodies affects cognitive benefit, glial activation and amyloid clearance in Alzheimer’s-like mice

Fig. 5

Ex vivo antibody-mediated phagocytosis assay demonstrates differences in plaque clearance based on antibody isotype. Unfixed, frozen plaque-rich tissue sections (20 μm) from 20-mo-old APPSWE/PS1ΔE9 Tg mice were pre-incubated with the following antibodies for 1 h: 07/1 IgG1, isotype control IgG1, 07/2a IgG2a, 07/2a-k IgG2a, or isotype control IgG2a. Following washing, the tissue was incubated with primary murine microglia (PMG) for 24 h and Aβ levels were determined. Representative photomicrographs of the hippocampus from each treatment group co-immunolabeled with a general Aβ marker, R1282, an activated microglial and macrophage marker, CD68 and a nucleic acid dye, DAPI (a-g) and showed decreased R1282 immunolabeling in the hippocampus of tissue pre-incubated with 07/2a IgG2a compared to isotype control (ah). pGlu-3 Aβ, Aβx-40, and Aβx-42 levels were also determined by ELISA (ik). Significant reductions in pGlu-3 Aβ were observed in tissue pre-incubated with 07/1 IgG1 and 07/2 IgG2a mAbs compared to their respective isotype controls (i). Tissue incubation with 07/2a IgG2a and 07/2a-k IgG2a resulted in decreased Aβx-40 levels compared to isotype control (j). There were no differences in Aβx-42 levels observed between groups (k). n = 3 per group. All data are expressed as the mean ± SEM. ANOVA with Neuman-Keuls post test: ***p < 0.001, **p < 0.01, and *p < 0.05 versus isotype control

Back to article page