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Fig. 9 | Alzheimer's Research & Therapy

Fig. 9

From: Nrf2 activation through the PI3K/GSK-3 axis protects neuronal cells from Aβ-mediated oxidative and metabolic damage

Fig. 9

Translational activation of the downstream Nrf2 targets SOD-1 and HO-1 by methazolamide, melatonin, and Trolox in SH-SY5Y cultures. Cells were treated with the Nrf2 activators MTZ, MEL, and Trolox in the presence/absence of Aβ42 (0–20 μM) followed by immunodetection of SOD1 and HO-1 expression by incubation with the pertinent primary antibodies and subsequent immunoreaction with Alexa-488 conjugated secondary antibodies. Green fluorescence highlights SOD-1 (a) and HO-1 (b) immunoreactivity; blue fluorescence depicts DAPI nuclear DNA counterstaining. Bar, 25 μm in all cases. The graphs in a and b depict respectively the quantitation of SOD-1 and HO-1 fluorescence signal in at least 400 cells utilizing ImageJ software and expressed in fold of control. Data is represented as mean ± SD; ****p < 0.0001

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