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Fig. 6 | Alzheimer's Research & Therapy

Fig. 6

From: Nrf2 activation through the PI3K/GSK-3 axis protects neuronal cells from Aβ-mediated oxidative and metabolic damage

Fig. 6

Lack of effect of methazolamide, melatonin, and Trolox on Aβ oligomerization. Oligomer Aβ immunoreactivity in cell culture supernatants from SH-SY5Y cells treated 24 h with Aβ42 (10 μM) in the presence/absence of MTZ (300 μM), MEL (100 μM), or Trolox (300 μM) was assessed by dot blot analysis. The respective supernatants of triplicate experiments were loaded onto the nitrocellulose membrane and probed with A11 anti-oligomer antibody, as described in the “Material and methods” section. Reactivity with 4G8 anti-Aβ antibody was used as a loading control. Right panel depicts the immunoreactivity of membranes loaded with freshly prepared synthetic Aβ42 (800 ng) as a negative control for oligomer formation. Histograms on the right panel illustrate the densitometric quantification of the dot blot A11 signal intensities normalized to the respective 4G8 controls; data are represented as mean ± SEM

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