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Fig. 4 | Alzheimer's Research & Therapy

Fig. 4

From: Nrf2 activation through the PI3K/GSK-3 axis protects neuronal cells from Aβ-mediated oxidative and metabolic damage

Fig. 4

Methazolamide, melatonin, and Trolox protect from Aβ-mediated ROS generation in SH-SY5Y. Following 24 h incubation with Aβ42 (0–25 μM) in the presence or absence of MTZ (300 μM), MEL (100 μM), and Trolox (300 μM), ROS-generated species were detected with CellROX (5 μM), and nuclei counterstained with Hoechst (1 μg/ml). Images depict CellRox fluorescence (red signal) and DAPI DNA counterstaining; bar, 25 μm. The graph on the right illustrates the quantitation of CellROX fluorescence values normalized to DAPI signal using ImageJ analysis software; data is represented as mean ± SEM. **p < 0.01 and ***p < 0.001

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