Fig. 3

Methazolamide, melatonin, and Trolox prevent Aβ42-induced mitochondrial dysfunction in human SH-SY5Y cells and rat primary cortical neurons. Both cells were grown in Seahorse XFe24 culture plates, incubated 24 h with increasing concentrations of Aβ42 in the presence or absence of MTZ (for SH-SY5Y (a) and primary neurons (b)), MEL (for SH-SY5Y (c) and primary neurons (d)), and Trolox (for SH-SY5Y (e) and primary neurons (f)). Cell bioenergetic profiles were analyzed with Cell Mito Stress Assay in a Seahorse XFe24 metabolic analyzer. Panels on the left illustrate the real-time kinetic rate of oxygen consumption (OCR) and indicate the injection time of the different respiratory chain modulators; right panels depict the test parameters calculated with Report Generator software. In all cases shown, OCR levels were normalized to DNA content assessed by Janus Green staining; bars illustrate means ± SEM from at least three to six independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001