Skip to main content
Fig. 2 | Alzheimer's Research & Therapy

Fig. 2

From: Nrf2 activation through the PI3K/GSK-3 axis protects neuronal cells from Aβ-mediated oxidative and metabolic damage

Fig. 2

Aβ42-mediated metabolic/bioenergetic changes in SH-SY5Y cells and rat primary cortical neurons. Both cell types were incubated 24 h in the presence of increasing concentrations of Aβ42 and analyzed through the use of Cell Mito Stress Assay in a Seahorse platform as described in the “Material and methods” section. Panels on the left illustrate the real-time kinetic rate of oxygen consumption (OCR) versus time and indicate the injection time of the different modulators of the respiratory chain. Right panels depict the test parameters calculated with Seahorse XF Report Generator software. In all cases shown, OCR levels were normalized to DNA content assessed by Janus Green staining; bar graphs illustrate means ± SEM from at least three to six independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001

Back to article page
\