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Fig. 10 | Alzheimer's Research & Therapy

Fig. 10

From: Nrf2 activation through the PI3K/GSK-3 axis protects neuronal cells from Aβ-mediated oxidative and metabolic damage

Fig. 10

Translational activation of the downstream Nrf2 targets SOD-1 and HO-1 by methazolamide, melatonin, and Trolox in primary cortical neurons. Cells were treated with the Nrf2 activators MTZ, MEL, and Trolox in the presence/absence of Aβ42 (0–1 μM) as in Fig. 9. Expression of SOD-1 (a) and HO-1 (b) was detected by immunocytochemical analysis using rabbit polyclonal anti-SOD-1 and mouse monoclonal anti-HO-1 primary antibodies followed by the pertinent Alexa-488 conjugated secondary antibodies. Green fluorescence highlights SOD-1 (a) and HO-1 (b) immunoreactivity; blue fluorescence depicts DAPI nuclear DNA counterstaining. Bar, 25 μm in all images. The graphs in a and b depict respectively the quantitation of SOD-1 and HO-1 fluorescence signal in 50–100 cells utilizing ImageJ software and expressed in fold of control. Data is represented as mean ± SD; ****p < 0.0001

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