Table 1 Proteins overrepresented in Aβ-enriched fraction and proteins overrepresented in non-AD samples. The MS/MS raw files were examined against the universal protein database UniProt (70,931 entries in January 2019) (http://www.uniprot.org), using the SEQUEST algorithm (Proteome Discoverer 1.4, Thermo Fisher Scientific). Fold of change; P value < 0.05. PSM peptide-spectrum matches, x̄ average, ND-AD not detected in Alzheimer extracts, ND-non-AD not detected in control extracts
Symbol | Entrez gene name | ID | PSM non-AD1 | PSM non-AD2 | PSM AD1 | PSM AD2 | x̄ non-AD | x̄ AD | Fold of change | p value |
|---|---|---|---|---|---|---|---|---|---|---|
A) Proteins overrepresented in AD enriched fractions | ||||||||||
GFAP | Glial fibrillary acidic protein | P14136 | 36 | 48 | 71 | 100 | 42 | 85.5 | 2.04 | 1E−4 |
MBP | Myelin basic protein | J3QL64 | 11 | 14 | 41 | 17 | 12.5 | 29 | 2.32 | 0.013 |
SH2D3C | SH2 domain-containing protein 3C (fragment) | Q5JU32 | 19 | 2 | 47 | 2 | 10.5 | 24.5 | 2.33 | 0.019 |
ANXA5 | Annexin A5 | P08758 | 0 | 0 | 4 | 15 | 0 | 9.5 | ND–non AD | 0.001 |
HIST1H2BK | Histone H2B type 1-K | O60814 | 0 | 0 | 9 | 1 | 0 | 5 | ND–non AD | 0.025 |
ANXA2 | Annexin A2 | H0YMM1 | 0 | 0 | 2 | 6 | 0 | 4 | ND–non AD | 0.045 |
B) Proteins overrepresented in non-AD enriched fractions | ||||||||||
COL6A3 | Collagen alpha-3(VI) chain | E7ENL6 | 13 | 28 | 0 | 7 | 20.5 | 3.5 | 5.86 | 6E−4 |
FGB | Fibrinogen beta chain | P02675 | 15 | 23 | 0 | 15 | 19 | 7.5 | 2.53 | 0.034 |
FN1 | Fibronectin | P02751 | 2 | 5 | 0 | 0 | 3.5 | 0 | ND–AD | 0.045 |