Fig. 9

siRNA repression of SAT1 reduces tau pathology in vitro. a Representative images and quantification of western blot analysis of C3 HeLa cells stably overexpressing tau (4R0N) following transfection with siRNA scramble negative control or siRNA SAT1 (n = 3). b One way analysis of variance (ANOVA) revealed an effect of group on monomeric Tau pSer356 (F(3, 8) = 4.743, p = .035), and post hoc comparisons revealed a significant decrease in response to siRNA SAT1 with and without DSPM (p = .029, p = .010, respectively), compared to siRNA scrambled control. c One way analysis of variance (ANOVA) revealed an effect of group on monomeric Tau pSer396 (F(3, 8) = 15.829, p = .001), and post hoc comparisons revealed a significant decrease in response to siRNA SAT1 with and without DSPM (p = .003, p = .001, respectively), compared to siRNA scrambled control. d One way analysis of variance (ANOVA) revealed no effect of group on monomeric total tau (F(3, 8) = 2.236, p = .161); however, post hoc comparisons revealed a significant decrease in response to siRNA SAT1 without DSPM (p = .05), compared to siRNA scrambled control. One way analysis of variance (ANOVA), followed by post hoc comparisons using Fishers PLSD, *p < .05. Data is represented by means ± S.E.M.