Fig. 4

Autoradiography of TSPO-binding sites. a Quantification of [³H]PK11195 binding in the neocortex of aging wild-type (WT) and APP_swe/PS1_dE9 transgenic (TG) mice. Values represent the mean-specific binding of [³H]PK11195 ± SEM in six animals/group. Levels of the translocator protein (TSPO) were increased by age in both TG and WT animals, but the increase was more prominent in APP_swe/PS1_dE9 mice. Significant differences between age-matched TG and WT animals are denoted by stars (^*). Age-dependent differences between groups of TG and WT animals in the frontal, parietal, and occipital cortices are denoted by hashes (^#) and daggers (^†), respectively. Two-way ANOVA analysis is shown, followed by Bonferroni post hoc tests. b Representative autoradiograms of [³H]PK11195-binding sites, presented at lateral 2.64–2.88 mm. The bar represents a scale of black and white image density, calibrated in fmol/mg of tissue equivalent. Levels of specific binding were calculated following subtraction of non-specific binding (NSB) from total binding images. NSB was determined with 10 μM unlabeled PK11195 and amounted to ~ 20% of total radioligand binding