Fig. 2

SAR255952 and SAR228810 protect neurons from Aβ oligomer neurotoxicity in vitro. Concentration-response effects of SAR228810 and SAR255952 on the loss of neurite network and the increase in caspase-3/7 enzymatic activity induced by oAβ42 in mouse primary neuronal cultures. a Cell images of MAP2 immunostaining in mouse primary neuronal cultures following a 48-h treatment with oligomeric amyloid β (oAβ)42 with or without anti-amyloid antibodies. b Quantification of the effects of anti-amyloid antibodies on the neurite network as measured by automated image analysis of MAP2 staining. c Effects of anti-amyloid antibodies on cell viability as measured by caspase-3/7 enzymatic activity. *p = 0.0023; **p < 0.0001; p = 0.0062; p = 0.0352, by two-tailed Dunnett’s test with respect to oAβ42. Horizontal axis represents concentration of respective mAbs in μg/ml. Horizontal black lines and error bars denote mean ± SEM data. Data are expressed as percentage of control group from five independent experiments. Dotted lines correspond to control values without oAβ42 (100%)