Fig. 6

DNA amyloid-β 1–42 (Aβ₄₂) immunization reduces total and phosphorylated tau in brains of triple-transgenic Alzheimer’s disease (3xTg-AD) mice. Quantitative enzyme-linked immunosorbent assay analyses for tau in detergent-soluble and nonsoluble fractions of brain lysates from 20-month-old 3xTg-AD mice. a Analysis of total concentrations of human tau. Blue bars show concentrations found in DNA Aβ₄₂ trimer-immunized mice, and yellow bars show concentrations found in brains of Aβ₄₂ peptide-immunized mice. Black bars show the values of age- and gender-matched 3xTg-AD control mice. The left-hand graph shows the analyses in detergent-soluble fractions from hemibrain lysates; the right-hand graph represents the analyses from nonsoluble fractions. b Analysis of tau phosphorylated at residue T231 (pT231), c Analysis of tau phosphorylated at residue S396 (pS396). d Analysis of tau phosphorylated at residue T181 (pT181). e Analysis of tau phosphorylated at residue S199 (pS199). All data are based on the analyses and comparison of 7 DNA Aβ₄₂ trimer-immunized mice, 9 Aβ₄₂ peptide-immunized mice, and 14 age- and gender-matched 3xTg-AD control mice. All samples were run in duplicates, and the assay was repeated twice. * p ≤ 0.05, ** p  ≤ 0.01, and *** p  ≤ 0.005 (Mann-Whitney U test)