Fig. 1
From: Dynamic changes of oligomeric amyloid β levels in plasma induced by spiked synthetic Aβ42
Detection of synthetic amyloid-β 1–42 peptide (Aβ42). a Freshly dissolved synthetic Aβ42 and double-mutant Aβ42 (mutAβ42) were visualized on the 4–12% aspartate PAGE gel by Coomassie blue staining and on the Western blot by FF51-horseradish peroxidase (HRP) antibody. b Standard curve for multimer detection system (MDS). Synthetic peptides were serially diluted and measured by 6E10/FF51-HRP MDS assay. Change of Aβ42 over different times (c and d). Aβ42 was incubated at 37 °C at the indicated time points. To measure amyloid-β oligomer (AβO), an MDS assay was used (c), and fibrillization of Aβ42 over time was measured by thioflavin T (ThT) binding assay (d). TEM images over time by Aβ42 in buffer vehicle are shown. Data are mean ± SD. RLU Relative luminescence units, RFU Relative fluorescence units