Fig. 7
From: Prevention of dendritic and synaptic deficits and cognitive impairment with a neurotrophic compound
Rescue of DG neurogenesis deficit after 9 month posttreatment with P021 in 3 × Tg-AD mice. After 9 months of treatment, P021- (Tg-AD-P021) and vehicle-treated (Tg-AD vh) mice were killed and their brains were analyzed for neurogenesis by doublecortin (DCX) and Ki67 immunohistochemical stainings. a DCX and TOPRO staining in wild-type (WT), 3 × Tg-AD-vh, and Tg-AD-P021mice; b Ki-67 and TOPRO staining in WT, 3 × Tg-AD-vh, and 3 × Tg-AD-P021 mice. c Densiometric quantification of the number of DCX-positive and Ki-67-positive cells. Labeled cells in the granule cell layer and the hilus of the DG of the hippocampus were manually quantified using a 40× oil objective of a Nikon 90i fluorescent microscope. The number of DCX-positive cells and Ki-67-positive cells was analyzed as the percent of control. Neurogenesis was deficient in the 3 × Tg-AD-vh and P021 was able to boost it in the P021-treated mice. Arrowheads indicate positive cells. **p < 0.01, ***p < 0.001, by one-way ANOVA post-hoc test. Scale bar = 50 μm. Quantification is shown as mean ± SD