Fig. 3

The antibody induced by EBY100-L2 vaccine (AOE1) specifically recognized β-amyloid peptide (Aβ) oligomers. After the last immunization, the sera from the mice immunized with AOE1 vaccine or Aβ₁₅ were diluted to 1:100 or 1:1000, respectively. The binding of antibodies induced by AOE1 vaccine or Aβ₁₅ to different Aβ isoforms was detected by enzyme-linked immunosorbent assay (a) or dot blot analysis (b), respectively. 7PA2 cell lysates mixed with Aβ monomer (c) and brain homogenate of APP/PS1 mice (d) were separated on SDS-PAGE gels, and then Western blot analysis was performed with sera from vaccine-immunized mice as primary antibodies. e Brain sections from APP/PS1 mice were incubated with sera of AOE1-treated mice and 4G8 and then stained with thioflavin S (ThS). Red arrows indicate the ThS-positive compact plaques. OD450 Optical density at 450 nm