Table 2 Summary of key studies on electrophysiological profiles of human iPSC-derived neurons
From: Connectivity and circuitry in a dish versus in a brain
| Â | Pre and colleagues, 2014 [9] | Tang and colleagues, 2013 [10] | Zhang and colleagues, 2013 [120] | ||||||
|---|---|---|---|---|---|---|---|---|---|
Method | Human iPSC neuronal differentiation via smad inhibition | Human iPSC-derived NPC neuronal differentiation | Human iPSC-iN cells (via Neurogenin-2 expression) | ||||||
Cell line | 7889O | WT126, WT33 | Not stated | ||||||
DIV | 31 to 38 | 41 to 45 | 55 | 55 | 42 | 60 | 42 | 60 | 21 |
AP firing (% of cells) | 20 | 48 | 60 | 86 | NQ | NQ | NQ | NQ | >95 |
Single (%) | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ |
Repetitive (%) | NQ | NQ | NQ | NQ | 16 | Â | 73 | Â | NQ |
Spontaneous AP (%) | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ |
n | 18 | 27 | 20 | 15 | 25 | NQ | 22 | NQ | 33 to 34 |
Rin (MΩ) | 2,500.0 | 2,200.0 | 1,700.0 | NQ | NQ | 695.0 | NQ | 302.0 | NQ |
Cm (pF) | NQ | NQ | NQ | NQ | NQ | 27.0 | NQ | 119.0 | NQ |
Ï„ (milliseconds) | 34.0 | 29.0 | 21.0 | NQ | NQ | NQ | NQ | NQ | NQ |
RMP (mV) | −35.0 | −38.0 | −49.0 | NQ | NQ | −44.0 | NQ | −59.0 | NQ |
n | 89 | 75 | 103 | NQ | NQ | 22 | NQ | 23 | NQ |
Spontaneous activity (%) | 11 | 16 | 21 | 27 | NQ | NQ | NQ | NQ | >95 |
Evoked potential (%) | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | >95 |
n | 8 | 6 | 20 | 15 | NQ | NQ | NQ | NQ | 33 to 34 |
Co-culture | x | Mouse glial cells | x | x | Mouse astrocytes | Mouse astrocytes | Mouse glial cells | Â | Â |