Figure 4
Ethosuximide and brivaracetam reduce spike-wave discharges in transgenic Alzheimer’s disease mice. Transgenic Alzheimer’s disease (AD) mice were given a single injection of 20 mg/kg phenytoin (PHT) (A, E), 200 mg/kg ethosuximide (ETX) (B, F), 20 mg/kg levetiracetam (LVT) (C, G) or 10 mg/kg brivaracetam (Briva) (D), followed by hourly quantification of spike-wave discharges (SWDs). For phenytoin, no reduction in SWDs at 0 to 5 hours were seen in either strain (A, E) (APP/PS1: −6% ± 17 (increased SWDs), P = 0.75; 3xTg-AD: −61% ± 76 (increased SWDs), P = 0.23). Ethosuximide strongly reduced SWDs at 0 to 5 hours in both strains (B, F) (APP/PS1: 93% ± 4, ***P < 0.0001; 3xTg-AD: 83% ± 5, ***P < 0.0001). Levetiracetam reduced SWDs at 0 to 5 hours in both strains (C, G) (APP/PS1: 45% ± 12, *P < 0.01; 3xTg-AD: 61% ± 24, **P = 0.002). Brivaracetam reduced SWDs in APP/PS1 mice by 41% ± 7, * P < 0.01, with an R 2 of 0.95. APP/PS1, n = 4; 3xTg-AD, n = 4. P-values were calculated by paired two-tailed Student’s t-test. n.s., Not significant.