Figure 3

The presence of spike wave discharges is not associated with changes in amyloid precursor protein metabolism or amyloid-β levels. In APP/PS1 mice, the presence of spike-wave discharges (SWDs) did not alter levels of soluble amyloid precursor protein (sAPP)-α (100 kDa marker), β-C-terminal fragment (β-CTF) (15 kDa marker) or amyloid-β (Aβ) monomers (5 kDa marker) by Western blot analysis (A, D). Actin loading control is indicated by the 37 kDa marker. In the same strain, Aβ plaque deposits were not altered by the presence of SWDs (B, C, E). Similarly, in 3xTg-AD mice, the presence of SWDs did not alter levels of sAPP-α or β-CTF as analyzed by Western blotting (F, I), nor did it alter levels of Aβ plaque (G, H, J). Data were analyzed by two-tailed Student’s t-test. APP/PS1, n = 11; 3xTg-AD, n = 11. +Indicates the presence of SWDs, and − indicates no SWDs. HC, Hippocampus; C, Cortex; PSEN, Presenelin; WT, Wild type.