Figure 3

Representative 2D DIGE gel of CSF proteins and PCA of brain proteins. (A) Low-abundance proteins from CSF of AD patients and control subjects were compared, with control samples labeled with Cy3 (Green) and AD samples labeled with Cy5 (Red). (B) Protein spots displaying significant different expression levels, which were successfully identified with mass spectrometry, are shown with the spot numbers automatically assigned by the DeCyder 2D software. (C) PCA analysis of all the proteins detected from brain tissues of AD patients and corresponding controls indicates that these are distinct experimental groups.