Crystal structure reveals conservation of amyloid-β conformation recognized by 3D6 following humanization to bapineuzumab

Table 2 Residues of the antibody contacting A β (that is, within 4 angstroms) in the structure of 3D6 with A β1-7

Aβ residue	Structure	VL contacts	VH contacts	Peptide contacts
D1	1-7	W89 G91 R96	Y95H S100a S100b	A2 E3 F4
D1	4HIX	W89 G91 R96	Y95H S100a S100b	A2 E3 F4
A2	1-7	G91 T92 H93 F94		D1 E3 F4 R5 H6
A2	4HIX	G91 T92 H93 F94		D1 E3 F4 R5 H6
E3	1-7	F94 R96	W47 S50 R52 Y58	D1 A2 F4 R5
E3	4HIX	F94 R96	W47 S50 R52 Y58	D1 A2 F4 R5
F4	1-7	R96	G33 M34 S50 I51 R52 Y95	D1 A2 E3 R5
F4	4HIX	R96	G33 M34 S50 I51 R52 Y95	D1 A2 E3 R5
R5	1-7	D27d D28 Y32 G91 T92	Y95	A2 E3 F4 H6
R5	4HIX	D27d D28 Y32 G91 T92	R52 Y95	A2 E3 F4 H6

Antigen contacting antibody residues revealed by the structure of 4HIX [24] is shown for comparison. Residues are numbered according to [36].

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