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Table 2 Squalene does not reverse the effects of Hexa-PAF upon Aβ 42 trafficking

From: Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases

 

Concentration of Aβ42, nM

 

Cell extracts

DRMs

Lysosomes

Control

8.7 ± 0.7

8.4 ± 0.6

0.4 ± 0.2

Hexa-PAF

8.5 ± 1.2

2.5 ± 0.9a

3 ± 0.6b

Squalene

8.5 ± 0.9

8 ± 0.4

0.5 ± 0.2

Hexa-PAF + squalene

8.8 ± 0.6

2.8 ± 0.8a

3 ± 0.4b

  1. Neurons were pre-treated with 1 μM Hexa-PAF, 5 μM squalene, or a mixture of Hexa-PAF and squalene and then incubated with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42 for 2 hours. The amounts of Aβ42 in whole cell extracts, detergent-resistant membranes (DRMs) (lipid rafts), and lysosomes were measured by enzyme-linked immunosorbent assay. Values are the mean concentration of Aβ42 ± standard deviation from triplicate experiments performed three times (n = 9). aAmounts of Aβ42 significantly lower than in control cells. bAmounts of Aβ42 significantly higher than in control cells. Hexa-PAF, 1-O-hexadecyl-2-acetyl-sn-glycerol-3-phospho-(N,N,N-trimethyl)-hexanolamine.