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Figure 6 | Alzheimer's Research & Therapy

Figure 6

From: Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases

Figure 6

Platelet-activating factor (PAF) causes the release of cholesterol in the endoplasmic reticulum (ER). (A) Neuronal extracts were separated by density gradient centrifugation and analyzed for cholesterol ester hydrolase (CEH) (□) and anti-acyl-coenzyme A:cholesterol acyltransferase (ACAT) (■). (B) The amount of cholesterol in ER fractions pre-treated with control medium (□), 20 μM diethylumbelliferyl phosphate (DEUP), or 2 μM PAF or pre-treated with DEUP and incubated with PAF (■). Values are mean ± standard deviation (SD) from triplicate experiments performed three times (n = 9). *Significantly less (P <0.05) cholesterol than ER fractions incubated with PAF. (C) The amount of cholesterol esters in ER fractions incubated with control medium (□), 20 μM DEUP, or 2 μM PAF or pre-treated with DEUP and incubated with PAF (■). Values are mean ± SD from triplicate experiments performed three times (n = 9). *Significantly more (P <0.05) cholesterol esters than ER fractions incubated with PAF. (D) Neurons treated with control medium () or 20 μM DEUP () were pulsed with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42 and incubated for 1 to 5 days as shown. Values are mean Aβ42 ± SD from triplicate experiments performed four times (n = 12). (E) The amount of Aβ42 in neurons pre-treated with control medium () or 20 μM DEUP (), pulsed with 100 nM synthetic Aβ42, and incubated for 1 to 5 days. Values are mean Aβ42 ± SD from triplicate experiments performed four times (n = 12).

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