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Figure 5 | Alzheimer's Research & Therapy

Figure 5

From: Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases

Figure 5

Platelet-activating factor (PAF) increased cholesterol in the endoplasmic reticulum (ER) of neurons. (A) Neuronal extracts were separated by density gradient centrifugation and analyzed for PAF receptor (□) and the ER marker Grp 78 (■). (B) The amounts of cholesterol in ER fractions incubated with control medium (□), 1 μM squalestatin, or 2 μM PAF or pre-treated with squalestatin and incubated with PAF (■) for 1 hour. Values are mean ± standard deviation (SD) from triplicate experiments performed three times (n = 9). *Concentration of cholesterol significantly higher (P <0.05) than in controls. (C) The amounts of cholesterol esters in ER fractions incubated with control medium (□), 1 μM squalestatin, or 2 μM PAF or pre-treated with squalestatin and incubated with PAF (■). Values are mean ± SD from triplicate experiments performed three times (n = 9). *Concentration of cholesterol esters significantly lower (P <0.05) than in controls.

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