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Figure 3 | Alzheimer's Research & Therapy

Figure 3

From: Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases

Figure 3

Platelet-activating factor (PAF) receptor antagonists increased the degradation of Aβ 42 . (A) Amounts of Aβ42 in neurons pre-treated with control medium (), 1 μM AACOCF3 (), or 1 μM MAFP (□), pulsed with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42, and incubated for 1 to 5 days. Values are mean ± standard deviation (SD) from triplicate experiments performed four times (n = 12). (B) Amounts of Aβ42 in neurons pre-treated with control medium (), 1 μM Hexa-PAF (), or 1 μM ginkgolide B (□), pulsed with 7PA2-CM containing 10 nM Aβ42, and incubated for 1 to 5 days. Values are mean ± SD from triplicate experiments performed four times (n = 12). (C) Neurons were incubated with 7PA2-CM containing 10 nM Aβ42. After 2 days, 1 μM Hexa-PAF () or control medium () was added and the amounts of Aβ42 were measured thereafter. Values are mean Aβ42 ± SD from triplicate experiments performed four times (n = 12). (D) Neurons were pre-treated with control medium (), 1 μM PAF (□), 1 μM AACOCF3 (), or a combination of 1 μM AACOCF3 and 1 μM PAF (■) and incubated with 7PA2-CM containing 10 nM Aβ42 for 1 to 5 days. Values are mean Aβ42 ± SD from triplicate experiments performed three times (n = 9). (E) The amount of Aβ42 in neurons pre-treated with control medium () or 1 μM Hexa-PAF (), pulsed with 100 nM synthetic Aβ42, and incubated for 1 to 5 days. Values are mean ± SD, from triplicate experiments performed four times (n = 12). AACOCF3, arachidonyl trifluoromethyl ketone; Hexa-PAF, 1-O-hexadecyl-2-acetyl-sn-glycerol-3-phospho-(N,N,N-trimethyl)-hexanolamine; MAFP, methyl arachidonyl fluorophosphonate.

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