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Figure 4 | Alzheimer's Research & Therapy

Figure 4

From: Calmodulin levels in blood cells as a potential biomarker of Alzheimer’s disease

Figure 4

Calmodulin content in freshly isolated peripheral blood mononuclear cells from control, mild cognitive impairment and Alzheimer’s disease patients. Peripheral blood mononuclear cells were isolated from freshly obtained blood from 15 control individuals, seven mild cognitive impairment (MCI) patients and 21 Alzheimer’s disease (AD) patients (Table 1, lower part) following density gradient centrifugation as described in Methods. Cells were washed twice in phosphate-buffered saline, lysed in ice-cold lysis buffer and subjected to immunoblot. A representative experiment is shown. Band intensity was measured and normalized by that of β-actin. To compare the results between experiments, the same standard sample (ST) was included in every western blot, and all values were referred to ST CaM levels. At least two different experiments were performed with each individual. Box plots represent CaM content in lymphoblasts from healthy, MCI and AD patients. (*p <0.05, **p <0.01 significantly different from control).

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